Influence of bloom number and plastifiers on gelatin matrices produced for enzyme immobilization

AUTOR(ES)

Kempka, A. P., Souza, S. M. A. G. Ulson de, Ulson de Souza, A. A., Prestes, R. C., Ogliari, D.

FONTE

Braz. J. Chem. Eng.

DATA DE PUBLICAÇÃO

2014-03

RESUMO

In this study the immobilization of lipase in matrices produced with gelatin with different Bloom values and with the addition of plastifiers was investigated to evaluate the influence of the Bloom value, as well as the capacity of the plastifiers to maintain the enzyme immobilized and the immobilization yield. The results indicated the need for crosslinking of the matrices with glutaraldehyde due to the high solubility in water, explained by the amino acid profile, which confirms the solubility of gelatin. Mannitol showed greater efficiency in the lipase immobilization, since it led to more porous structures and more uniform pores. These structures were also influenced by the gelatin concentration; greater concentrations associated with intermediate concentrations of plastifier led to matrices with a greater immobilization yield (87.92%). The X-ray diffraction analysis revealed that the structure of the immobilization matrices was partially crystalline.

Documentos Relacionados

• Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents
• Preparation of silica with controlled pore sizes for enzyme immobilization
• Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles
• Methacrylated Chitosan Based UV Curable Support for Enzyme Immobilization
• On the Use of a TiO2 Sol-Gel for Horseradish Peroxidase Enzyme Immobilization for Development of an Amperometric Biosensor