Influence of 2% chlorhexidine gel on pH, calcium release and ability of reducing endotoxin of calcium hydroxide / Avaliação in vitro da influencia da clorexidina 2% gel no pH, liberação de calcio e ação sobre endotoxinas do hidroxido de calcio

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The aim of the present study was to evaluate the influence of 2% chlorhexidine gel (CHX) on chemical properties of calcium hydroxide and on its ability of reducing endotoxic content of root canals when used as intracanal medicament. The amount of calcium released from the pastes was assessed by atomic absorbance spectophotometry and the pH was verified in pHmeter. The tested periods were 24 hours and 7, 15 and 30 days. For the endotoxin quantification assay, single rooted teeth were previously inoculated with endotoxins and then with intracanal medication. The medicaments were placed inside the root canal of 30 teeth, (GI - Ca(OH)2 + 0.9% saline solution, GII - Ca(OH)2 + CHX and GIII - CHX), and were removed after 14 days, with copiously irrigation. The removed solution was submitted to the chromogenic quantitative endotoxin test QCL-1000 Statistical analysis was performed with ANOVA and Tuckey Test (p<0.05). For calcium release analysis there was no statistical difference among the groups with Ca(OH)2 associated or not with CHX in the periods of 24 hours, 7 days and 30 days. However, after 15 days, lower values were observed for Ca(OH)2+ saline solution. In the groups that contained calcium hydroxide associated or not with CHX, an increase in ion release was observed from 24h to 7 days. However there was a decrease in the period of 15 days for the Ca(OH)2+ saline solution. An alkaline pH was observed in all groups that contained calcium hydroxide (p>0.05). The highest pH values were observed after 30 days for the association between Ca(OH)2 + saline solution. The lowest values of pH were observed for 2% chlorhexidine gel, in all periods. A higher increase of pH was stated between 24 h and 7 days for Ca(OH)2+ saline solution and Ca(OH)2+ CHX, and remained unaltered until the end of the experiment for both pastes. A higher reduction in the endotoxin content was observed in the CHX group (91.63%), followed by Ca(OH)2+ CHX (88,76%) and Ca(OH)2 + saline solution (82.13%). There were no statistical difference between 2% chlorhexidine gel and its association with calcium hydroxide over the endotoxin content reduction inside the root canal. According to the results, it can be concluded that 2% chlorhexidine gel did not interfere in the chemical properties of calcium hydroxide. Moreover, chlorhexidine was also able to improve calcium hydroxide properties for reducing the endotoxic content of root canals in vitro

ASSUNTO(S)

endodontia endodontics

ACESSO AO ARTIGO

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