Induction of SOS functions by alkaline intracellular pH in Escherichia coli.
AUTOR(ES)
Schuldiner, S
RESUMO
Alkalinization of intracellular pH (pHi) causes an increase in UV resistance in wild-type and pH-sensitive mutant (DZ3) cells of Escherichia coli. Utilizing cells transformed with a plasmid (pA7) which bears the uvrA promoter fused to galK galactokinase structural gene, it was shown that alkaline pHi leads to an increase in the specific activity of galactokinase. This effect was not displayed in a mutant bearing a recA-insensitive lexA gene, nor in cells harboring a plasmid (pA8) in which the galK is fused to a lexA-insensitive uvrA promoter. Hence, the effects of pHi on cells functions may involve the lexA product of the SOS system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213574Documentos Relacionados
- pH dependence of CheA autophosphorylation in Escherichia coli.
- Mu d-directed lacZ fusions regulated by low pH in Escherichia coli.
- pH dependence and gene structure of inaA in Escherichia coli.
- ATP hydrolysis during SOS induction in Escherichia coli.
- An inhibitor of SOS induction, specified by a plasmid locus in Escherichia coli.