Indel-Based Evolutionary Distance and Mouse–Human Divergence
AUTOR(ES)
Ogurtsov, Aleksey Y.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
We propose a method for estimating the evolutionary distance between DNA sequences in terms of insertions and deletions (indels), defined as the per site number of indels accumulated in the course of divergence of the two sequences. We derive a maximal likelihood estimate of this distance from differences between lengths of orthologous introns or other segments of sequences delimited by conservative markers. When indels accumulate, lengths of orthologous introns diverge only slightly slower than linearly, because long indels occur with substantial frequencies. Thus, saturation is not a major obstacle for estimating indel-based evolutionary distance. For introns of medium lengths, our method recovers the known evolutionary distance between rat and mouse, 0.014 indels per site, with good precision. We estimate that mouse–human divergence exceeds rat–mouse divergence by a factor of 4, so that mouse–human evolutionary distance in terms of selectively neutral indels is 0.056. Because in mammals, indels are ∼14 times less frequent than nucleotide substitutions, mouse–human evolutionary distance in terms of selectively neutral substitutions is ∼0.8.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=509270Documentos Relacionados
- Impact of the Presence of Paralogs on Sequence Divergence in a Set of Mouse-Human Orthologs
- Mouse-Human Heterokaryons Support Efficient Human Immunodeficiency Virus Type 1 Assembly
- Uniparental propagation of mitochondrial DNA in mouse-human cell hybrids.
- Identifying Novel Genes for Atherosclerosis through Mouse-Human Comparative Genetics
- Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids.