Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
AUTOR(ES)
Laurito, Magdalena
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
28/11/2019
RESUMO
Sand fly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve this kind of problem. This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed well supported clusters and were diagnosed as primary species. These sequences are useful tools for molecular identification of the sand flies of the New World.
Documentos Relacionados
- Phlebotomine sand flies in the State of Piauí, Brazil (Diptera: Psychodidae: Phlebotominae)
- Modification of Disney trap for capture of sand flies (Diptera: Psychodidae: Phlebotominae)
- Ecology of Sand Flies (Diptera: Psychodidae: Phlebotominae) in the North of the State of Mato Grosso, Brazil
- Reorganization and cleanness of peridomiciliar area to control sand flies (Diptera, Psychodidae, Phlebotominae) in South Brazil
- Diversity of sandflies (Psychodidae: Phlebotominae) captured in sandstone caves from Central Amazonia, Brazil