Increased production of beta-lactamase under anaerobic conditions in some strains of Escherichia coli.
AUTOR(ES)
Rashtchian, A
RESUMO
A simple biological assay to detect beta-lactamase activity exhibited by selected cultures of Escherichia coli was used to test enzyme production in cells incubated aerobically and anerobically. Anaerobic incubation resulted in increased size of zones of drug inactivation by some beta-lactamase-producing strains. The beta-lactamase activity of cell lysates was determined iodometrically for aerobically and anaerobically grown cells. The specific beta-lactamase activity for anaerobically grown cells was three to five times greater than for aerobically grown cells. Beta-lactamase production was determined to be constitutive in all strains and to be plasmid mediated, as demonstrated by transfer to E. coli K-12 by conjugation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=352951Documentos Relacionados
- Plasmid-determined beta-lactamase indistinguishable from the chromosomal beta-lactamase of Escherichia coli.
- Beta-lactamase and penicillinacylase coexistence in Escherichia coli.
- In vivo regulation of chromosomal beta-lactamase in Escherichia coli.
- Lincomycin stimulates synthesis of TEM-2 beta-lactamase by Escherichia coli.
- Beta-Lactamase Activity in Anaerobic Bacteria
