Increased c-fos mRNA Expression By Human Fibroblasts Contracting Stressed Collagen Matrices
AUTOR(ES)
Rosenfeldt, Hans
FONTE
American Society for Microbiology
RESUMO
We studied early changes in gene expression during fibroblast contraction of stressed collagen matrices. The level of c-fos mRNA increased dramatically and peaked 50 to 60 min after matrix contraction was initiated. This response did not require serum and could not be accounted for simply by disruption of the actin cytoskeleton. Increased c-fos mRNA levels required Ca2+ influx but not the cyclic AMP or extracellular signal-regulated kinase (ERK 1/2) signaling pathways, both of which are activated when fibroblasts contract stressed collagen matrices. The levels of two other immediate-early genes, fosb and c-jun, also increased transiently after fibroblast contraction, whereas the levels of fra-1, fra-2, c-myc, and the transcription factor NF-κB remained the same, indicating that fibroblast contraction caused changes in a selective group of genes. The increase in c-fos mRNA during contraction of stressed collagen matrices may reflect a unique role for c-fos in mechanoregulated events at the end of wound repair.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=110645Documentos Relacionados
- Posttranscriptional regulation of c-fos mRNA expression.
- Regulation of c-fos gene expression in hamster fibroblasts: initiation and elongation of transcription and mRNA degradation.
- c-fos mRNA expression in macrophages is downregulated by interferon-gamma at the posttranscriptional level.
- Heparin inhibits c-fos and c-myc mRNA expression in vascular smooth muscle cells.
- Augmentation of c-fos mRNA expression by activators of protein kinase C in fresh, terminally differentiated resting macrophages.
