Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin.
AUTOR(ES)
Amicosante, M
RESUMO
Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=228003Documentos Relacionados
- Improvement of Mycobacterium tuberculosis detection in sputum using DNA extracted by sonication
- Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization.
- Detection of Mycobacterium tuberculosis in sputum samples by polymerase chain reaction using a simplified procedure.
- Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification of Mycobacterium tuberculosis.
- Inactivation of Mycobacterium tuberculosis for DNA Typing Analysis
