Inactivation of catalase monolayers by irradiation with 100 keV electrons.
AUTOR(ES)
Hahn, M
RESUMO
A catalase monolayer adsorbed on a layer of arachidic acid deposited on a solid support was irradiated with 100 keV electrons simulating the conditions of electron microscopic imaging. Effective doses were calculated taking into account the angular and energy distribution of backscattered electrons. Enzymatic inactivation was chosen as the criterion for damage and was monitored by a rapid and quantifiable but nevertheless sensitive assay. Dose-response curves revealed that inactivation is a one-hit-multiple-target phenomenon, which is consistent with biochemical evidence for a cooperative function of subunits. The experimentally determined target size coincides fairly well with both calculated cross sections for inelastic interactions based on the atomic composition of catalase and with calculated cross sections for ionizing events based on the chemical bonds involved. This legitimates both types of calculations even for complex biomolecules.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=336011Documentos Relacionados
- Estudo do alcance de elétrons com energias entre 110 eV e 50 KeV
- DNA strand breaks measured within 100 milliseconds of irradiation of Escherichia coli by 4 MeV electrons.
- Anteprojeto de um implantador de ions para 40 KeV
- Construção de um acelerador de elétrons de 20KeV: aplicação ao estudo dos polímeros.
- Alcances de ions energéticos (10 a 390 keV) implantados em silício amorfo