IN VIVO Function of Rare G6pd Variants from Natural Populations of DROSOPHILA MELANOGASTER
AUTOR(ES)
Eanes, Walter F.
RESUMO
From 1981 to 1983, 15,097 X-chromosomes were genetically extracted from a number of North American populations of D. melanogaster and were electrophoretically screened for rare mobility and activity variants of glucose-6-phosphate dehydrogenase (G6PD). Overall, 13 rare variants were recovered for a frequency of about 10-3. Eleven variants affect electrophoretic mobility and are apparently structural, and two variants exhibit low G6PD activity. One low activity variant is closely associated with a P-element insertion at 18D12-13—all of the variants were subjected to the previously described genetic scheme used to identify relative in vivo activity differences between the two common electrophoretic variants associated with the global polymorphism. Most of the rare variants exhibit apparent in vivo activities that are similar to one or the other of the common variants, and these specific rare variants appear to be geographically widespread. Several variants have significantly reduced function. All of the variants were measured for larval specific activity for G6PD as a first measure of in vitro activity. It appears that specific activity alone is not a sufficient predictor for G6PD in vivo function.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1202862Documentos Relacionados
- Amino Acid Polymorphism and Rare Electrophoretic Variants of G6pd from Natural Populations of Drosophila Melanogaster
- Direct Measurement of in Vivo Flux Differences between Electrophoretic Variants of G6pd from Drosophila Melanogaster
- Viability Interactions, IN VIVO Activity and the G6pd Polymorphism in DROSOPHILA MELANOGASTER
- Comparison of in Vitro and in Vivo Activities Associated with the G6pd Allozyme Polymorphism in Drosophila Melanogaster
- Evidence for adaptive evolution of the G6pd gene in the Drosophila melanogaster and Drosophila simulans lineages.