In Vivo Detection of Membrane Injury at Freezing Temperatures

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The release of hydrogen cyanide from Amelanchier alnifolia was monitored at 30 C and −10 C following lethal freezing at both slow and fast rates. Assuming that hydrogen cyanide release indicates membrane damage, it was concluded that during a fatal freeze-thaw cycle membrane damage occurred during cell contraction and, therefore, was not dependent upon membrane area expansion during thawing.

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