In Vivo Conversion of 5-Oxoproline to Glutamate by Higher Plants 1
AUTOR(ES)
Mazelis, Mendel
RESUMO
l-(U-14C)-5-oxoproline (pyrollidone carboxylic acid or pyroglutamic acid) was infiltrated into detached leaves of a number of species and incubated for 1 to 6 hours. In every case, conversion to labeled glutamate and glutamine was observed. The amount converted varied from 1 to 64% of the total label fed depending on the species. The ratio of glutamate-14C to glutamine-14C ranged from 5 in Vicia faba to 1 in sugar beet. This ratio could be affected by preinfiltrating various compounds before allowing the uptake of the 5-oxoproline. When l-methionine-dl-sulfoximine was prefed to sugar beet leaves, the glutamate-glutamine ratio increased from 1 to 10. Prior treatment of V. faba leaves with azaserine resulted in essentially only labeled glutamine being recovered. Preinfiltration with NaF or ATP gave similar results in that the glutamate-glutamine ratio was greatly decreased. The results are consistent with glutamate being produced from the 5-oxoproline and then being converted to glutamine.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541968Documentos Relacionados
- Formation of 5-Oxoproline from Glutathione in Erythrocytes by the γ-Glutamyltranspeptidase-Cyclotransferase Pathway
- Accumulation of 5-Oxoproline in Mouse Tissues After Inhibition of 5-Oxoprolinase and Administration of Amino Acids: Evidence for Function of the γ-Glutamyl Cycle*
- HYDROGEN ION CONCENTRATION IN RELATION TO ABSORPTION OF INORGANIC NUTRIENTS BY HIGHER PLANTS1
- Metabolism of Hydrogen Cyanide by Higher Plants 1
- Attempts to Detect Cyclic Adenosine 3′:5′-Monophosphate in Higher Plants by Three Assay Methods 12