In vitro evaluation of the antimicrobial activity of Castor-oil based irrigants / Avaliação in vitro da atividade antimicrobiana de ésteres orgânicos derivados de óleo de mamona

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The accumulation and metabolism of microorganisms in the mouth are considered the main causes of the caries, periodontal diseases, endodontic infections, periimplantar infections and stomatitis. Solutions with antimicrobial activity, together with mechanical therapies, can be used in the treatment of periodontal diseases and endodontic infection to eliminate or reduce the number of microorganisms and thus collaborate with the eradication of the infectious process. Different types of solutions have been indicated and used in the irrigation of periodontal pockets and radicular canals. The research of new agents and solutions is justified because the importance of finding a solution with the highest antimicrobial action and the least possible toxicity. The purpose of this study was to evaluate in vitro the antimicrobial action of solutions derived from the castor oil on the microorganisms Candida albicans, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus e Staphylococcus epidermidis , using as positive control the solution of 2% Chlorhexidine digluconate. The experiment used the methods of diffusion in agar and microdilution. In the diffusion agar test the solution of 2% Chlorhexidine digluconate presented inhibition halos against all tested microorganisms. The ester derived from the castor bean oil in its concentrated form inhibited the growth of Gram-positive bacteria Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis. Endoquil ® and Perioquil® exhibited halos against Gram-positive bacteria Enterococcus faecalis.In the microdilution test the same bacteria were used to assess the maximum inhibitory dilution (DIM) of the solutions studied. In plate of 96 holes the solution of 2% Chlorhexidine digluconate inhibited the growth of all microorganisms tested with DIM ranging from 128 to Enterococcus faecalis to 32768 for Staphylococcus epidermidis. The ester derived from the castor bean oil concentrate presented antimicrobial activity on gram-positive bacteria Enterococcus faecalis (DIM 128), Staphylococcus aureus (DIM 1024), Staphylococcus epidermidis (DIM 32768) and the yeast Candida albicans (DIM 512). Endoquil ® and Perioquil ® did not exhibit antimicrobial action.Transferring a rate from each microdilution hole for tubes, it can be observated that, in some cases, the DIM was lower than that observed in the plate. In these situations it is concluded that the solutions presented in lower dilutions, a bactericidal action and in high dilutions, a bacteriostatic action.

ASSUNTO(S)

microbiologia microbiology Óleo de rícino castor oil

ACESSO AO ARTIGO

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