In vitro-derived leukemic erythroid cell lines induced by a raf- and myc-containing retrovirus differentiate in response to erythropoietin.
AUTOR(ES)
Klinken, S P
RESUMO
In vitro infection of murine fetal liver cells with a retrovirus containing v-raf and v-myc oncogenes has produced continuous lines of immature erythroid cells that are leukemogenic. These cells synthesized a factor that stimulated their growth in vitro before autonomous variants emerged. Approximately 1000 high-affinity erythropoietin receptors could be detected per cell, and the hormone induced terminal differentiation in these cells. The lines were generated at an extremely low frequency (approximately 1 in 10(7) cells), suggesting that the combination of raf and myc is insufficient to develop erythroid cell lines and that additional events are necessary for transformation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=282487Documentos Relacionados
- Characterization of a myc-containing retrovirus generated by propagation of an MH2 viral subgenomic RNA.
- Transforming variants of the avian myc-containing retrovirus FH3 arise prior to phenotypic selection.
- Polycythemia vera. The in vitro response of normal and abnormal stem cell lines to erythropoietin.
- Excystation of in vitro-derived Giardia lamblia cysts.
- Transforming potential of a myc-containing variant of feline leukemia virus in vitro in early-passage feline cells.