In Vitro and In Vivo Characterization of Helicobacter hepaticus Cytolethal Distending Toxin Mutants
AUTOR(ES)
Young, Vincent B.
FONTE
American Society for Microbiology
RESUMO
Helicobacter hepaticus expresses a member of the cytolethal distending toxin (CDT) family of bacterial cytotoxins. To investigate the role of CDT in the pathogenesis of H. hepaticus, transposon mutagenesis was used to generate a series of isogenic mutants in and around the cdtABC gene cluster. An H. hepaticus transposon mutant with a disrupted cdtABC coding region no longer produced CDT activity. Conversely, a transposon insertion outside of the cluster did not affect the CDT activity. An examination of these mutants demonstrated that CDT represents the previously described granulating cytotoxin in H. hepaticus. Challenge of C57BL/6 interleukin 10−/− mice with isogenic H. hepaticus mutants revealed that CDT expression is not required for colonization of the murine gut. However, a CDT-negative H. hepaticus mutant had a significantly diminished capacity to induce lesions in this murine model of inflammatory bowel disease.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=387909Documentos Relacionados
- Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter hepaticus
- Characterization of a Haemophilus ducreyi Mutant Deficient in Expression of Cytolethal Distending Toxin
- Prevalence of Cytolethal Distending Toxin Production in Periodontopathogenic Bacteria
- Characterization of Cytolethal Distending Toxin of Campylobacter Species Isolated from Captive Macaque Monkeys†
- Introns in the Cytolethal Distending Toxin Gene of Actinobacillus actinomycetemcomitans
