In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves

AUTOR(ES)

Schmelzer, Elmon

RESUMO

Methods involving in situ RNA hybridization, immunohistochemistry, and microspectrophotometry of individual cells were used to localize the mRNA encoding chalcone synthase (the key enzyme of flavonoid biosynthesis), the enzyme protein, and the biosynthetic end products in cross sections of parsley leaves (Petroselinum crispum). The light-dependent, sequential occurrence of all three components was restricted to epidermal cells. The results are in agreement with the putative function of flavonoids (transcriptionally inducible, UV-protective pigments) and suggest that all biosynthetic steps occur in those cells in which the products accumulate.

Documentos Relacionados

• Ultraviolet light-induced crosslinking of mRNA to proteins.
• Light-Induced Changes in Hydrogen, Calcium, Potassium, and Chloride Ion Fluxes and Concentrations from the Mesophyll and Epidermal Tissues of Bean Leaves. Understanding the Ionic Basis of Light-Induced Bioelectrogenesis1
• Glutathione and a UV Light–Induced Glutathione S-Transferase Are Involved in Signaling to Chalcone Synthase in Cell Cultures
• Psoralens potentiate ultraviolet light-induced inhibition of epidermal growth factor binding.
• LIGHT-INDUCED PARAMAGNETISM IN CHLOROPLASTS*