In situ localization and molecular characterization of Pleurotus ostreatus endosymbiont bacteria / Localização in situ e caracterização molecular da bactéria endossimbionte de Pleurotus ostreatus

AUTOR(ES)

Ricardo Yara

DATA DE PUBLICAÇÃO

2006

RESUMO

The fungus Pleurotus ostreatus, which belongs to white rot basidiomycete group, is a widely cultivated mushroom; this species has high productivity and rusticity, besides its use in biobleaching and bioremediation processes. This biotechnological potential justifies microbial interaction studies between this fungi and others microorganisms. In P. ostreatus mycelia, it has been observed pleomorphic bacteria growing on agar media. This research describes several assays to confirm bacterial presence in this sample. Therefore, the full-cycle rRNA analysis (described for unculturable or fastidious microorganism), ultrastructure and basic microbiology approaches were employed. Basic microbiology approaches indicated slow growing bacteria, which grown faster near to fungi colonies in solid media amended with Tween 80 or Tween 20 (co-culture system). Ultrastructure studies confirm the presence of intracellular and extracellular pleomorphic bacteria. The full-cycle rRNA analysis started with 16S rDNA amplification and sequencing. This approach demonstrated a relation between these bacteria with Burkholderia cepacia complex. By bioinformatics analysis was determinate which DNA probes can be use to identified this bacterial group. The last step for full-cycle rRNA analysis was applying fluorescent in situ hybridization (FISH). This technique confirmed the relationship between 16S rDNA bacterial sequence and bacterial forms. This is the first time that a pleomorphic bacteria from B. cepacia complex is found associated with P. ostreatus.

ASSUNTO(S)

edible mushroom endossimbiose fungo xilófago microscopia eletrônica cogumelo comestível endosybiont xylophagous fungus bactérias aeróbicas gram-negativa gram negative aerobic bacterium molecular genetics electron microscopy genética molecular microscopia de fluorescência fluorescence microscopy

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