In situ localisation of Yersinia enterocolitica by catalysed reported deposition signal amplification.

AUTOR(ES)

Odinot, P T

RESUMO

AIM: The sensitive detection of pathogenic Yersinia enterocolitica in paraffin embedded tissue sections by in situ hybridisation (ISH). METHODS: Y enterocolitica infected cell lines, rat spleens, and patient biopsy specimens were used to compare conventional ISH, immune fluorescence assay (IFA) detection, and catalysed reporter deposition (CARD) signal amplification ISH. RESULTS: CARD-ISH was shown to be more sensitive then conventional ISH and had a comparable sensitivity to IFA. In contrast to IFA, CARD-ISH preserved good tissue morphology. CONCLUSIONS: CARD-ISH appeared to be a fast and sensitive ISH method for detecting Y enterocolitica in routinely processed tissue sections. Application of this method allows the combination of routine detection and cellular localisation of the pathogen within the infected tissue.

Documentos Relacionados

• Detection of immunoglobulin light chain mRNA by in situ hybridisation using biotinylated tyramine signal amplification.
• Human papilloma virus detection by in situ hybridisation signal amplification based on biotinylated tyramine deposition.
• Human papilloma virus detection by in situ hybridisation signal amplification based on biotinylated tyramine deposition
• In cell amplification.
• Assessment of mycobacterial viability by RNA amplification.