In situ hybridization of hepatitis C virus RNA in liver cells of an experimentally infected rhesus macaque
AUTOR(ES)
Majerowicz, Selma, Grief, Christopher, Ferguson, Debora, Airano, Renata C, Baptista, Marcia L, Pinto, Marcelo A, Barth, Ortrud Monika
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2004-10
RESUMO
The liver tissue of a rhesus macaque inoculated with hepatitis C virus (HCV) has been analyzed for the presence of HCV RNA using the technique of in situ hybridization, both at light and electron microscopy levels. The animal was inoculated by the intrasplenic route using a HCV infected autogenic hepatocyte transplant. The serum sample used to infect the hepatocyte cells was characterized by polymerase chain reaction technique and shown to be positive for HCV RNA, genotype 3 with 10(7) RNA copies/ml. In situ hybridization was performed using a complementary negative strand probe made with the specific primer. We were able to detect and localize viral RNA in altered membranes of the rough endoplasmic reticulum of infected liver cells, showing evidence of virus replication in vivo.
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