Improving Yield of 1,3-Diglyceride by Whole-Cell Lipase from A. Niger GZUF36 Catalyzed Glycerolysis via Medium Optimization
AUTOR(ES)
Li, Cuiqin, Li, Lanxiang, Zhou, Huanjing, Xia, Chaoshuang, He, Laping
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2015-02
RESUMO
1,3-Diglyceride (1,3-DG) has attracted considerable attention as a healthful food oil component. In this study, we reported a novel method to synthesis of 1,3-DG via glycerolysis of triglyceride (TG) catalyzed by whole-cell lipase from a new isolated strain, Aspergillus niger GZUF36. The glycerolysis products include major diglyceride and minor monoglyceride. To improve yield of 1,3-DG, the optimized medium for the strain was obtained by combination of single-factor experiments, Plackett-Burman (PB), the steepest ascent and Box-Behnken design (BBD). Using the optimized medium, yield of 1,3-DG synthesized by the whole-cell lipase was 26.90 mol% and 1.42-fold yield was improved. The optimized medium lays the foundation for further studies of the reaction system for synthesis of 1,3-DG. The whole-cell catalyzed-glycerolysis process represents a significant advance toward achieving economical production of 1,3-DG at industrial scale.
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