Improvements in the Growth of BHK-21 Cells in Submerged Culture
AUTOR(ES)
Radlett, P. J.
RESUMO
A medium is described which will support the submerged culture of BHK-21 cells to 6.5 × 106 to 7 × 106 cells/ml in volumes up to 30 liters. Shaken-flask cultures were used to determine nutrient deficiencies in depleted medium. The final medium became limited by glutamine at 6.5 × 106 to 7 × 106 cells/ml, but increasing the glutamine concentration failed to improve cell yields. The value of the polyol Pluronic F68 as a protective substance is illustrated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=376357Documentos Relacionados
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