Improved Method for Purification of Bacterial DNA from Bovine Milk for Detection of Brucella spp. by PCR

AUTOR(ES)

Romero, C.

FONTE

American Society for Microbiology

RESUMO

Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.

Documentos Relacionados

• Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.
• Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes.
• Identification of Brucella by Ribosomal-Spacer-Region PCR and Differentiation of Brucella canis from Other Brucella spp. Pathogenic for Humans by Carbohydrate Profiles
• Twelve-Hour PCR-Based Method for Detection of Salmonella spp. in Food
• Simplified method for recovery and PCR detection of Cryptosporidium DNA from bovine feces.