Improved Double-Vial Radiorespirometric Technique for Mineralization of 14C-Labeled Substrates †

AUTOR(ES)

Coveney, Michael F.

RESUMO

New procedures which simplify sample preparation and improve counting efficiency were developed for double-vial radiorespirometry. Under certain conditions, efficiency of fluor-NaOH-impregnated wicks was not stable, but was adversely affected by water vapor, NaOH concentration, and CO2 loading. Glass fiber wick material treated with a methanolic fluor-NaOH solution showed improved 14C counting efficiency (58%) compared with previous methods.

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