Improved Colorimetric Determination of Cell Wall Chitin in Wood Decay Fungi
AUTOR(ES)
Chen, George C.
RESUMO
The Svennerholm modification of the Elson-Morgan method for glucosamine analysis was evaluated for its applicability to the rapid determination of chitin in wood decay fungi. The evaluation included extent of chromogen interference, sensitivity, color stability, and hydrolysis conditions for maximum release of glucosamine from fungal cell walls. With our further modification, the Svennerholm method was shown to be suitable for rapid quantitative determination of fungal chitin without chromatographic separation of hydrolysate chromogens.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=239259Documentos Relacionados
- Wood-decay fungi (Agaricomycetes, Basidiomycota) in three physiognomies in the Savannah region in Brazil
- Host-exclusivity and host-recurrence by wood decay fungi (Basidiomycota - Agaricomycetes) in Brazilian mangroves
- Characterization of Aspergillus nidulans mutants deficient in cell wall chitin or glucan.
- Catalase-Aminotriazole Assay, an Invalid Method for Measurement of Hydrogen Peroxide Production by Wood Decay Fungi
- QUANTITATIVE ESTIMATION OF CHITIN IN FUNGI1