Improved Adaptation to Cold-Shock, Stationary-Phase, and Freezing Stresses in Lactobacillus plantarum Overproducing Cold-Shock Proteins

AUTOR(ES)

Derzelle, Sylviane

FONTE

American Society for Microbiology

RESUMO

We have investigated the effect of overproducing each of the three cold shock proteins (CspL, CspP, and CspC) in the mesophilic lactic acid bacterium Lactobacillus plantarum NC8. CspL overproduction transiently alleviated the reduction in growth rate triggered by exposing exponentially growing cells to cold shock (8°C), suggesting that CspL is involved in cold adaptation. The strain overproducing CspC resumed growth more rapidly when stationary-phase cultures were diluted into fresh medium, indicating a role in the adaptation and recovery of nutritionally deprived cells. Overproduction of CspP led to an enhanced capacity to survive freezing.

Documentos Relacionados

• Sensitivity of Escherichia coli to Cold-Shock during the Logarithmic Growth Phase 1
• The role of the 5'-end untranslated region of the mRNA for CspA, the major cold-shock protein of Escherichia coli, in cold-shock adaptation.
• RNA-binding strategies common to cold-shock domain- and RNA recognition motif-containing proteins
• Adaptive, or Stationary-Phase, Mutagenesis, a Component of Bacterial Differentiation in Bacillus subtilis
• Cold-shock regulation of the Arabidopsis TCH genes and the effects of modulating intracellular calcium levels.