Immunospecific T-lymphocyte stimulation by membrane proteins from Francisella tularensis.
AUTOR(ES)
Sandström, G
RESUMO
Membranes from a capsule-deficient mutant of the live-vaccine strain of Francisella tularensis (F. tularensis LVS) were treated with N-lauroyl sarcosinate (Sarkosyl; CIBA-GEIGY Corp., Summit, N.J.). When the Sarkosyl-insoluble fraction was heated in the presence of sodium dodecyl sulfate and mercaptoethanol and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, several polypeptides were distinguished. Four major polypeptides were eluted from the gel, each of which stimulated lymphocytes from tularemia-vaccinated individuals but not from nonvaccinated individuals. The stimulation occurred mainly in T lymphocytes. Radioactive labeling of surface proteins of the capsule-deficient bacteria indicated that at least two of the four polypeptides originated from outer membrane proteins. The results suggest that several membrane proteins of F. tularensis LVS induce a specific T-lymphocyte response.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266051Documentos Relacionados
- Stimulation of human lymphocytes by a vaccine strain of Francisella tularensis.
- Cellular fatty acid composition of Francisella tularensis.
- T-lymphocyte subpopulations in uveitis.
- Use of immunoelectron microscopy to demonstrate Francisella tularensis.
- T-cell-independent resistance to infection and generation of immunity to Francisella tularensis.
