Immunohistological identification of interleukin-1 activated chondrocytes.

AUTOR(ES)

Dingle, J T

RESUMO

Interleukin-1 activated chondrocytes have been identified in pig articular cartilage by immunolocalisation using a polyclonal antiserum recognising cytokine induced surface epitopes. Although chondrocytes with membrane staining were noted in all zones of the cartilage, only a proportion of the cells were positive within the range of antiserum dilution used (1:100-1:2000). Maximum staining was found after four days' treatment of cultured cartilage with pig IL-1 alpha, though weak staining was just detectable after treatment for one day at high antiserum concentration. Immunoreactivity was also shown in cartilage which had been cultured with human recombinant IL-1 alpha.

Documentos Relacionados

• Interleukin-1 beta-modulated gene expression in immortalized human chondrocytes.
• Effects of interleukin-1 beta on insulin-like growth factor-I autocrine/paracrine axis in cultured rat articular chondrocytes.
• Identification of an adhesion factor for chondrocytes.
• Production of interleukin-1 receptor antagonist by human articular chondrocytes
• Cartilage homeoprotein 1, a homeoprotein selectively expressed in chondrocytes.