"Retroposon" insertion into the cellular oncogene c-myc in canine transmissible venereal tumor.
AUTOR(ES)
Katzir, N
RESUMO
We examined by Southern blotting the state of the cellular oncogene c-myc in the dog transmissible venereal tumor. The tumor DNA contains a 16.8-kilobase pair (kbp) rearranged c-myc fragment in addition to the normal 15-kbp and 7.5-kbp fragments. We compared the structure of the cloned rearranged c-myc (re-myc) with that of a cloned normal c-myc and found that the rearrangement was due to the insertion of a 1.8-kbp DNA upstream to the first exon of c-myc. The inserted DNA is flanked by 10-base-pair direct repeats and contains a dA-rich tail, suggesting its origin from mRNA. Partial sequence of the inserted element showed 62% homology with the primate interdispersed Kpn I repetitive element. These results provide an example for the behavior of repetitive DNA sequences like the Kpn I family, as movable elements that can transpose nearby to oncogenes or other structural genes and perhaps affect their activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=397192Documentos Relacionados
- Identity of rearranged LINE/c-MYC junction sequences specific for the canine transmissible venereal tumor.
- Posttranscriptional regulation of cellular gene expression by the c-myc oncogene.
- C-myc oncogene expression in anal squamous neoplasia.
- Diverse karyotypic abnormalities of the c-myc locus associated with c-myc dysregulation and tumor progression in multiple myeloma
- Transcriptional activation of the translocated c-myc oncogene in Burkitt lymphoma