Immunogenicity of Plague Vaccines in Mice and Guinea Pigs
AUTOR(ES)
von Metz, E.
RESUMO
The median effective doses (ED50) of 28 lots of killed Pasteurella pestis strain 195/P vaccine were determined in mice and guinea pigs. Mice were injected with vaccine alone, whereas guinea pigs received vaccine suspended in incomplete Freund's adjuvant. Potency ratios of vaccines were obtained by comparing the ED50 of the test with that of a reference vaccine. Mean potency ratios of 1.82 ± 0.50 in mice and 3.22 ± 0.56 in guinea pigs were obtained, and the difference between these means was significant, P = <0.01. The number of organisms in the challenge dose did not significantly affect the ED50 of a vaccine in guinea pigs. However, irrespective of vaccinating route, nearly 1,000 times as much vaccine was required in the absence of adjuvant as in its presence to produce comparable protective indexes in the guinea pig. The response of guinea pigs did not offer any improvement over mice in evaluating the efficacy of plague vaccines.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=377380Documentos Relacionados
- Comparative Efficacy and Immunogenicity of Replication-Defective, Recombinant Glycoprotein, and DNA Vaccines for Herpes Simplex Virus 2 Infections in Mice and Guinea Pigs
- INDUCTION OF ACQUIRED RESISTANCE IN GUINEA PIGS WITH DEFATTED MYCOBACTERIUM TUBERCULOSIS VACCINES
- INFLUENCE OF GUINEA PIG PLASMA FACTORS ON PHAGOCYTOSIS OF PASTEURELLA PESTIS: II. Plasma from Plague-Infected Guinea Pigs
- Pathological features of experimental gonococcal infection in mice and guinea pigs.
- Experimental Chagas' disease in complement-deficient mice and guinea pigs.