Immunoexpression of cleaved caspase-3 shows lower apoptotic area indices in lip carcinomas than in intraoral cancer
AUTOR(ES)
LEITE, Ana Flávia Schueler de Assumpção, BERNARDO, Vagner Gonçalves, BUEXM, Luisa Aguirre, FONSECA, Eliene Carvalho da, SILVA, Licínio Esmeraldo da, BARROSO, Danielle Resende Camisasca, LOURENÇO, Simone de Queiroz Chaves
FONTE
J. Appl. Oral Sci.
DATA DE PUBLICAÇÃO
2016-08
RESUMO
ABSTRACT Objective This study aimed to evaluate apoptosis by assessing cleaved caspase-3 immunoexpression in hyperplastic, potentially malignant disorder (PMD), and malignant tumors in intraoral and lower lip sites. Material and Methods A retrospective study using paraffin blocks with tissues from patients with inflammatory fibrous hyperplasia (IFH), actinic cheilitis, oral leukoplakia, lower lip and intraoral squamous cell carcinoma (SCC) was performed. The tissues were evaluated by immunohistochemical analysis with anti-cleaved caspase-3 antibody. Apoptotic area index was then correlated with lesion type. Results From 120 lesions assessed, 55 (46%) were cleaved caspase-3-positive. The SCC samples (n=40) had the highest apoptotic area indices (n=35; 87.5%). Significant differences were detected between SCCs and PMDs (p=0.0003), as well as SCCs and IFHs (p=0.001), regarding caspase-3 immunopositivity. Carcinomas of the lower lip had lower apoptotic area indices than intraoral cancer (p=0.0015). Conclusions Cleaved caspase-3 immunoexpression showed differences in oral SCCs and PMDs and demonstrated a distinct role of apoptosis in carcinogenesis of intraoral and lower lip cancer. In future, the expression of cleaved caspase-3 with other target molecules in oral cancer may be helpful in delineating the prognosis and treatment of these tumors.
Documentos Relacionados
- A non-apoptotic function of caspase-3 in pharmacologically-induced differentiation of K562 cells
- Evaluation of Bcl-2, Bcl-x and Cleaved Caspase-3 in Malignant Peripheral Nerve Sheath Tumors and Neurofibromas
- Francisella tularensis Induces Extensive Caspase-3 Activation and Apoptotic Cell Death in the Tissues of Infected Mice▿
- Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas
- Immunoexpression of human leukocyte antigen-DR in actinic cheilitis and lower lip squamous cell carcinoma