Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection

AUTOR(ES)

Eickhoff, Christopher S, Giddings, Olivia K, Yoshida, Nobuko, Hoft, Daniel F

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2010-08

RESUMO

The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.

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