Immobilization of Glucose Isomerase-Containing Streptomyces phaeochromogenes Cells in Fine-Particle Form
AUTOR(ES)
Kumakura, Minoru
RESUMO
A new preparation method for immobilizing Streptomyces phaeochromogenes cells in fine-particle form was investigated using radiation-induced polymerization at low temperatures with previously salted out hydrophilic monomers. Using this method, it was found that the glucose isomerase activity of the immobilized cell particles was markedly higher than that of immobilized cells in block form obtained without salting out of the monomer. The diameter of the particles was varied by changing the irradiation temperature or the concentrations of monomer and salt. The magnitude of the enzymatic activity increased with decreasing particle diameter. Km values of the immobilized cell particles were close to that of the intact cell. These facts suggested that the cells were trapped on the surface of the particle.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=243206Documentos Relacionados
- Evaluation of the anisotropy field for fine-particle systems from low-field thermomagnetic curves
- Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes1
- D-Glucose Isomerase: Constitutive and Catabolite Repression-Resistant Mutants of Streptomyces phaeochromogenes
- Purification, Immobilization, and Some Properties of Glucose Isomerase from Streptomyces flavogriseus†
- Production of Glucose Isomerase by Streptomyces flavogriseus†
