IL-4 enhances proliferation and mediator release in mature human mast cells

AUTOR(ES)

Bischoff, S. C.

FONTE

The National Academy of Sciences

RESUMO

Tissue mast cells (MC) are recognized as key effector cells of immediate-type allergic reactions releasing inflammatory mediators and cytokines on stimulation with antigen, but they also might be involved in IgE-independent inflammatory and tissue repair processes. The mechanism of human MC regulation in tissue is not fully understood. Here, we show that IL-4, in synergy with stem cell factor (SCF), regulates the function of purified human MC isolated from intestinal tissue. Whereas SCF induced only marginal proliferation of MC cultured in vitro up to 4 weeks, addition of IL-4 and SCF strongly increased the proliferation rate. Moreover, IL-4, which by itself had no visible effect on human MC, enhanced the release of histamine, leukotriene C4, and IL-5 in MC triggered by IgE receptor crosslinking. The IL-4 effects occurred in a dose-dependent fashion (ED50 = 100 pg/ml) and could be totally blocked by a competitive IL-4 receptor antagonist. Our data indicate that IL-4 is an important regulator of human MC function and suggest that mature MC retain the capacity to proliferate in a particular tissue environment.

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