Identification of the merR gene of R100 by using mer-lac gene and operon fusions.

AUTOR(ES)

Foster, T J

RESUMO

Transcriptional (operon) and translational (gene) fusions between the R100 merR gene and lacZ were constructed in vitro in a pBR322 plasmid carrying the mer genes derived from plasmid R100. The translational fusions were oriented in the opposite direction to and divergently from the merTCAD genes. This shows that the reading frame previously thought to be merR was incorrect. Expression of the gene fusion was repressed in trans by a compatible plasmid carrying the R100 merR+ gene, as was a similarly oriented transcriptional fusion. In contrast, expression of beta-galactosidase by the lac fragment located at the same site but in the opposite orientation was at a lower level and was not repressed by merR+.

Documentos Relacionados

• Characterization of fusions between the lac operon and the ilv gene cluster in Escherichia coli: ilvC-lac fusions.
• Identification and characterization of starvation-regulated genetic loci in Salmonella typhimurium by using Mu d-directed lacZ operon fusions.
• Some mercurial resistance plasmids from different incompatibility groups specify merR regulatory functions that both repress and induce the mer operon of plasmid R100.
• Identification of Shigella invasion genes by isolation of temperature-regulated inv::lacZ operon fusions.
• Mercury operon regulation by the merR gene of the organomercurial resistance system of plasmid pDU1358.