Identification of Gardnerella (Haemophilus) vaginalis.
AUTOR(ES)
Piot, P
RESUMO
Different tests for the identification of Gardnerella (Haemophilus) vaginalis and for its differentiation from catalase-negative unclassified coryneforms from the vagina were evaluated on over 200 bacterial strains, with special emphasis on optimal test conditions. A presumptive identification of G. vaginalis in the clinical laboratory can be made on the basis of colonial morphology, clear beta-hemolysis with diffuse edges on human blood bilayer-Tween agar, a negative catalase test, and typical cell morphology in the Gram stain. This procedure will correctly identify 90 to 98% of suspect colonies of G. vaginalis with human blood bilayer-Tween agar as primary isolation medium. Useful additional reactions for the confirmation of G. vaginalis include positive hippurate and starch hydrolysis, positive alpha-glucosidase but negative beta-glucosidase tests, the production of acid from glucose and maltose but not from mannitol, and susceptibility to disks containing metronidazole, nitrofurantoin, sulfonamides, and bile.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272015Documentos Relacionados
- Rapid microbiochemical method for identification of Gardnerella (Haemophilus) vaginalis.
- Selective differential human blood bilayer media for isolation of Gardnerella (Haemophilus) vaginalis.
- Sodium polyanetholesulfonate in the identification of Gardnerella vaginalis.
- Biotypes of Gardnerella vaginalis.
- Antimicrobial susceptibilities of Gardnerella vaginalis.