Identification of Bacillus subtilis genes for septum placement and shape determination.
AUTOR(ES)
Levin, P A
RESUMO
The Bacillus subtilis divIVB1 mutation causes aberrant positioning of the septum during cell division, resulting in the formation of small, anucleate cells known as minicells. We report the cloning of the wild-type allele of divIVB1 and show that the mutation lies within a stretch of DNA containing two open reading frames whose predicted products are in part homologous to the products of the Escherichia coli minicell genes minC and minD. Just upstream of minC and minD, and in the same orientation, are three genes whose products are homologous to the products of the E. coli shape-determining genes mreB, mreC, and mreD. The B. subtilis mreB, mreC, and mreD genes are the site of a conditional mutation (rodB1) that causes the production of aberrantly shaped cells under restrictive conditions. Northern (RNA) hybridization experiments and disruption experiments based on the use of integrational plasmids indicate that the mre and min genes constitute a five-cistron operon. The possible involvement of min gene products in the switch from medial to polar placement of the septum during sporulation is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=207347Documentos Relacionados
- The divIVB region of the Bacillus subtilis chromosome encodes homologs of Escherichia coli septum placement (minCD) and cell shape (mreBCD) determinants.
- Order of expression of genes affecting septum location during sporulation of Bacillus subtilis.
- Identification of two distinct Bacillus subtilis citrate synthase genes.
- Identification of Two myo-Inositol Transporter Genes of Bacillus subtilis
- Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.