Identification of Bacillus anthracis by rpoB Sequence Analysis and Multiplex PCR

AUTOR(ES)

Ko, Kwan Soo

FONTE

American Society for Microbiology

RESUMO

Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. thuringiensis, 1 of B. mycoides, and 1 of B. megaterium were amplified and sequenced. The determined rpoB sequences (318 bp) of the 10 B. anthracis strains, including five Korean isolates, were identical to those of Ames, Florida, Kruger B, and Western NA strains. Strains of the “B. cereus group” were separated into two subgroups, in which the B. anthracis strains formed a separate clade in the phylogenetic tree. However, B. cereus and B. thuringiensis could not be differentiated. Sequence analysis confirmed the five Korean isolates as B. anthracis. Based on the rpoB sequences determined in the present study, multiplex PCR generating either B. anthracis-specific amplicons (359 and 208 bp) or cap DNA (291 bp) in a virulence plasmid could be used for the rapid differential detection and identification of virulent B. anthracis.

Documentos Relacionados

• Utilization of the rpoB Gene as a Specific Chromosomal Marker for Real-Time PCR Detection of Bacillus anthracis
• rpoB Sequence Analysis of Cultured Tropheryma whippelii
• rpoB Gene Sequence-Based Identification of Staphylococcus Species
• Sequence and analysis of the rpoB gene of Mycobacterium smegmatis.
• Species Identification of Mycobacteria by PCR-Restriction Fragment Length Polymorphism of the rpoB Gene