Identification, characterization, and distribution of secretin immunoreactivity in rat and pig brain.
AUTOR(ES)
O'Donohue, T L
RESUMO
Secretin immunoreactivity was detected in the central nervous system of the rat and pig with a highly specific radioimmunoassay. The secretin immunoreactivity in the rat and pig brain and duodenum extracts was fractionated by using a reverse-phase high-pressure liquid chromatographic system. The immunoreactive secretin from pig brain and duodenum coeluted precisely with synthetic porcine secretin. However, immunoreactive secretin extracted from rat brain and duodenum eluted slightly before porcine secretin. These data suggest a slight difference in the structure of rat and pig secretin. The detection of secretin in the brain lays the groundwork for studies to determine the role of the peptide in central nervous system function.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320380Documentos Relacionados
- Glucocorticoid receptor immunoreactivity in monoaminergic neurons of rat brain.
- Cholecystokinin octapeptide-like immunoreactivity: histochemical localization in rat brain.
- Extraction and immunochemical characterization of cholecystokinin-like peptides from pig and rat brain.
- Identification of insulin in rat brain.
- Distribution of galanin immunoreactivity in the respiratory tract of pig, guinea pig, rat, and dog.