Identification and radiochemical purification of the recA protein of Escherichia coli K-12.
AUTOR(ES)
McEntee, K
RESUMO
The product of the recA gene of E. coli has been identified by labeling proteins synthesized in UV-treated cells after infection with specialized transducing phages carrying the recA gene. Following infection of UV-treated cells by lambda precA, which carries the recA+ gene, a major protein with a molecular weight of 43,000 is detected on polyacrylamide gels containing sodium dodecyl sulfate. This protein is also made after infection of suppressing hosts by lambda precA99, which carries an amber recA- mutation, but is not synthesized after infection of nonsuppressing hosts by this transducing phage. A spontaneous recatrevertant of lambda preca99 induces synthesis of this protein after infection of a nonsuppressing host. The product of the recA gene is a soluble protein found in a complex with a molecular weight of approximately 150,000 after mild detergent lysis of cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=431291Documentos Relacionados
- Genetic characteristics of new recA mutants of Escherichia coli K-12.
- Isolation of protease-proficient, recombinase-deficient recA mutants of Escherichia coli K-12.
- RecA protein acts at the initiation of stable DNA replication in rnh mutants of Escherichia coli K-12.
- recA (Srf) suppression of recF deficiency in the postreplication repair of UV-irradiated Escherichia coli K-12.
- Two-component suppression of recF143 by recA441 in Escherichia coli K-12.