Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
AUTOR(ES)
Ghorbanpur, M, Zare Mirakabadi, A, Zokaee, F, Zolfagarrian, H
FONTE
Journal of Venomous Animals and Toxins including Tropical Diseases
DATA DE PUBLICAÇÃO
05/02/2010
RESUMO
An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83%. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.
Documentos Relacionados
- Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys
- Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms
- Purification and partial characterization of phospholipases A2 from Bothrops asper (barba amarilla) snake venom from Chiriguaná (Cesar, Colombia)
- The Taipan snake venom time: a new test for lupus anticoagulant.
- Pharmacological and partial biochemical characterization of Bmaj-9 isolated from Bothrops marajoensis snake venom
