Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain.
AUTOR(ES)
MacKinnon, M G
RESUMO
The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS1476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-L-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=164010Documentos Relacionados
- Characterization of vanY, a DD-carboxypeptidase from vancomycin-resistant Enterococcus faecium BM4147.
- VanD-Type Vancomycin-Resistant Enterococcus faecium and Enterococcus faecalis
- VanD-Type Vancomycin-Resistant Enterococcus faecium 10/96A
- Large Conjugative vanA Plasmids in Vancomycin-Resistant Enterococcus faecium
- High Prevalence of VanB2 Vancomycin-Resistant Enterococcus faecium in Taiwan