Identificação e caracterização parcial de albuminas e globulinas de tres cultivares brasileiros de Phascolus vulgaris

AUTOR(ES)
DATA DE PUBLICAÇÃO

1993

RESUMO

Seeds of Phaseolus vulgaris cv. Aeté-3, Coiano precoce and Carioca were analysed in different aspects: contents of the different protein fractions of albumins and globulins, electrophoretic banding patterns, amino acid composition, evaluation of the range of precipitaiton of major vicilins, and their nutritional values. The degree of glycosilation, carboydrate content and in vitro enzymatic hydrolysis patterns were also analysed for faseolin. Several methods were used for the extraction, separation and analysis of the different protein fractions, e.g. precipitation using buffers; salts and differencial pH; dialysis; exclusion, affinity and liquid chromatography; SDS-PACE; densitometry; enzymatic, colorimetric and immunological reactions. The cultivars Aeté and Carioca were found to be similar in electrophoretic patterns and contents of albumins, globulins, lectins and legumins. The patterns of faseolin of these cultivars were identical, having one major and three minor bands in a proportion of 5:2: 1:2. They presented small seeds and were classified as "Sanilac". They are phylogeneticaly very similar but unlike Coiano. However, Lys and carboydrates of faseolin were higher in Carioca than the others. Coiano showed a very different electrophoretic pattern of faseolin, having two major bands in a proportion of 2:2:1:0,1, with bigger seeds than the others. Coiano had a pattern similar to "Contender". It was also different in lectin and legumin contents. It presented a higher carboydrate content than Aeté and higher Met and Cys levels than the others. All subunits of faseolin and the protein like-Iegumin of Aeté, Coiano and Carioca were glycosylated but the degree of glycosilation was indistinct. Faseolin of the three cultivars was hydrolysated by trypsin that acts in the central portion of the molecule, producing polypeptides with M s. around 20 kgfmol. The a subunit seemed the first to be degraded and in the case of Coiano. this subunit was hydrolysed at the carboxyl end, too. Faseolin from Coiano was more resistant to proteolytic attack than the others of Aeté and Carioca. The presence oí common antigenic determinants of faseolin between Aeté. Coiano and Carioca was determined. Some aspects like the high sulfur amino acids and Lys and the low hemoaglutin activity (lectins) suggest that Coiano might have a higher nutritional potencial than Aeté and Carioca

ASSUNTO(S)

proteinas feijão eletroforese

ACESSO AO ARTIGO

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