Identificação de interatores putativos envolvidos na localização de proteínas de duplo direcionamento em Arabidopsis thaliana / Identification of putative interactors involved in the localization of dual-targeted proteins in Arabidopsis thaliana

AUTOR(ES)

Larissa Spoladore

DATA DE PUBLICAÇÃO

2011

RESUMO

Most organellar proteins are nuclear encoded, synthesized in the cytosol and then targeted to their destination. Specific subcellular targeting is conducted by a complex machinery for the specific localization of the proteins, which includes targeting sequences, cytosolic proteins and specific organelar receptors. However, little is known about the process that happens from the synthesis of a protein and the transport to its final destination. Most organellar proteins contain the information for their localization in the N-terminal sequence. Many studies have searched to characterize the steps involved in protein targeting, from the early stages after its synthesis to the cytosolic factors regulating its correct localization. Post-translational modifications, 5-UTR regions, the C-terminal extension on the protein, alternative transport pathways and proteinprotein interactions may influence the subcellular location of some proteins. The use of biomolecular netwoks has become one of the main focus of systems biology studies and possess a major potential in the discovery of several biological processes, such as protein-protein interactions (PPI). Dual-targeted (DT) proteins in Arabidopsis thaliana were analyzed through a PPI network, and the proteins displaying interactions with DT proteins were selected by their funtion and location. The selected proteins were analyzed for their eventual role in the subcellular targeting of other proteins. Screenings in yeast two-hibrid assays were performed for the genes GRF9 (14-3-3) and ATH7 (h-type thioredoxin). The results for GRF9 include a peroxidase (PRXR1) and dihydrolipamide acetyltransferase. The results for ATH7 include glutamine synthetase (AT5G35630.3). Combination of in silico analysis with yeast two-hibrid screenings provide new perspectives for understanding the control of subcellular localization.

ASSUNTO(S)

chloroplasts clonagem cloning cloroplastos interaction of plant proteins. linhagem celular mitocôndrias vegetais papaverales papaverales plant mitochondria proteínas de plantas - interação.

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