IdentificaÃÃo e avaliaÃÃo da presenÃa de PapilomavÃrus bovino (BPV) em sangue de bovinos e cultura de linfÃcitos em uma fazenda leiteira do estado de Pernambuco

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Bovine papillomaviruses (BPVs), family Papillomaviridae, act as causal agent of warts, bladder and digestive tract cancers, causing significant losses to the cattle economy. Ten types of BPVs have been well characterized (BPV-1-10). This study has as main objective to evaluate sequences of BPV genomes in blood samples collected in a dairy farm in Pernambuco and in respective peripheral lymphocyte cultures, to determine the BPV 1, 2 or 4 presence and their simultaneous presence. The technique of the polymerase chain reaction (PCR) was used for viral diagnosis, using specific primers for types 1, 2 and 4, targeted to genes L1, L2 and E7, respectively. Confirmation of the amplified products was performed with enzymatic digestion and posterior sequencing. Viral sequences were detected in all the animals, regardless of the apparent presence of papillomas. The types 1 and 2 were detected directly from blood samples and in lymphocyte cultures and the type 4 was not detected in any samples. The positive results were confirmed by enzymatic digestion. The viral presence in blood corroborates studies that discuss BPV dissemination through blood while the detection in culture suggests viral maintenance in this system. The sequencing of some positive samples for BPV-1 suggests a new viral variant, compared to GenBank. In conclusion, the results obtained in this work generate more knowledge about BPV in Pernambuco, more specifically in RibeirÃo. Larger studies are necessary to include other farms of the State contributing to evaluation of BPV occurrence in this State.

ASSUNTO(S)

papilomavÃrus bovino (bpv) bovine papillomaviruses papillomavirus digestÃo enzimÃtica polymerase chain reaction - pcr genetica reaÃÃo em cadeia da polimerase (pcr) sequencing

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