Hyper-inducible expression system for streptomycetes
AUTOR(ES)
Herai, Sachio
FONTE
National Academy of Sciences
RESUMO
Streptomycetes produce useful enzymes and a wide variety of secondary metabolites with potent biological activities (e.g., antibiotics, immunosuppressors, pesticides, etc.). Despite their importance in the pharmaceutical and agrochemical fields, there have been no reports for practical expression systems in streptomycetes. Here, we developed a “PnitA-NitR” system for regulatory gene expression in streptomycetes based on the expression mechanism of Rhodococcus rhodochrous J1 nitrilase, which is highly induced by an inexpensive and safe inducer, ε-caprolactam. Heterologous protein expression experiments demonstrated that the system allowed suppressed basal expression and hyper-inducible expression, yielding target protein levels of as high as ≈40% of all soluble protein. Furthermore, the system functioned in important streptomycete strains. Thus, the PnitA-NitR system should be a powerful tool for improving the productivity of various useful products in streptomycetes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=521115Documentos Relacionados
- A steroid-inducible gene expression system for plant cells.
- Development of a temperature-inducible expression system for Streptomyces spp.
- Hyper Friburgo: um sistema hyper texto baseado em agentes inteligentes para informações turísticas
- Development of a Sensitive Gene Expression Reporter System and an Inducible Promoter-Repressor System for Clostridium acetobutylicum
- Control of ion channel expression for patch clamp recordings using an inducible expression system in mammalian cell lines