Hybridization of labeled RNA to DNA in agarose gels.

AUTOR(ES)

Shinnick, T M

RESUMO

Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA. RNA-DNA hybrids are detected by autoradiography of the gel. Comparison of band patterns of the autoradiogram and the ethidium bromide stained gel allows the identification of the DNA fragment which is complementary to the RNA probe. The technique is rapid, easy and inexpensive. It is sensitive enough to detect individual genes in a mixture of fragments produced by restriction enzyme digestion of complex cellular DNA. We have used this technique to determine which of the Hin III and Eco R1 fragments of phi80d3ilv+su+7 and E. coli DNAs contain the 5S, 16S and 23S ribosomal RNA (rRNA) genes of E. coli.

Documentos Relacionados

• Purification of DNA fragments from lyophilized agarose gels.
• Rat hemoglobin interferes with DNA migration in agarose gels.
• A method for the recovery of DNA from agarose gels.
• Electrophoretic properties of the scrapie agent in agarose gels.
• A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.