Human telomeres are attached to the nuclear matrix.
AUTOR(ES)
de Lange, T
RESUMO
This report shows that human telomeres are tightly associated with the nuclear matrix. Telomere attachment is observed in several cell types and in all stages of interphase. Mapping experiments show that telomeres are anchored via their TTAGGG repeats; a subtelomeric repeat located immediately proximal to the telomeric TTAGGG repeats is quantitatively released from the nuclear matrix by restriction endonuclease cleavage. TTAGGG repeats introduced at chromosome-internal sites by DNA transfection do not behave as matrix attached loci, suggesting that the telomeric position of the repeats is required for their interaction with the nuclear matrix. These findings are consistent with the idea that telomeres function as a nucleoprotein complex.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=556504Documentos Relacionados
- Replication forks are associated with the nuclear matrix.
- V-myc- and c-myc-encoded proteins are associated with the nuclear matrix.
- Mathematical model to predict regions of chromatin attachment to the nuclear matrix.
- Human papillomavirus 16 E7 protein is associated with the nuclear matrix.
- Replication origins are attached to the nuclear skeleton.