Human p53 inhibits growth in Schizosaccharomyces pombe.
AUTOR(ES)
Bischoff, J R
RESUMO
Overexpression of wild-type p53 in mammalian cells blocks growth. We show here that the overexpression of wild-type human p53 in the fission yeast Schizosaccharomyces pombe also blocks growth, whereas the overexpression of mutant forms of p53 does not. The p53 polypeptide is located in the nucleus and is phosphorylated at both the cdc2 site and the casein kinase II site in S. pombe. A new dominant mutation of p53, resulting in the change of a cysteine to an arginine at amino acid residue 141, was identified. The results presented here demonstrate that S. pombe could provide a simple system for studying the mechanism of action of human p53.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=369581Documentos Relacionados
- Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe.
- Malate transport in Schizosaccharomyces pombe.
- Induction of mitotic segregation with p-fluorophenylalanine in Schizosaccharomyces pombe.
- A mechanosensitive ion channel in Schizosaccharomyces pombe.
- Trehalose-6-P synthase is dispensable for growth on glucose but not for spore germination in Schizosaccharomyces pombe.