Homogeneous assays based on deoxyribozyme catalysis

AUTOR(ES)

Stojanovic, Milan N.

FONTE

Oxford University Press

RESUMO

We report herein the first homogeneous assays based on the ribonuclease activity of a deoxyribozyme. The previously reported deoxyribozyme was covalently modified with biotin and used to assay biotin-binding interactions through changes in fluorescence upon substrate turnover. Deoxyribozymes with fluorescence-based reporting have the potential to serve as general analytical tools.

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