HistoquÃmica e anÃlise digital de imagens em neoplasias cutÃneas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

This work evaluated alterations in the expression of carbohydrates and the density of tumour cells and Langerhans cells (LC) using lectin histochemistry, immunohistochemistry and computer image analysis in neoplasic cutaneous tissues. Biopsies of basal cell carcinoma (BCC, n=35), epidermoid carcinoma (EpC, n= 18), tricoepithelioma (TE, n=12), keratoacanthoma (KA, n=19), seborrheic keratosis (SK, n=16) and actinic keratosis (AK, n=18) were used. Patients were from both Sex and the average age was of 59.7 year-old. Thelectins Concanavalin A (Con A), Wheat germ agglutinin (WGA), Peanut agglutinin (PNA), Ulex europaeus agglutinin (UEA-I) and Tetragonolobus purpurea agglutinin (LTA) were used conjugated with peroxidase. In the immunohistochemistry study the S-100 protein was used to analyse the epidermal LC. The results showed that in the benign lesions, KA presented a pattern of aberrant expression of glucose/mannose, α-fucose e D-galactose residues, evidenced by the intense by Con A (94.7%), LTA (84,2%) and PNA (89,4%), respectively. The malign tumours presented distinct patterns of stainning when compared to the benign lesions. It was not observed significant variantions LC population among the malign tumours BCC(23.25Â5.81) e EpC (20.88Â4.24). However, it was observed a higher number of LC in studied benign lesions AK (102,04Â17,11), TE (79,74Â9,35), SK (122,38Â9,92) and KA (110,62Â31,4), when compared to the malign tumours and their normal counterpart (p<0.05). LC did not show significant difference related to the area and volume of LC in both benign and malign tissue. Despite the qualitative and quantitative data obtained in this study it is observed that biochemical and cell number alterations ocurred becoming important addictional parameters for distinction of histological features of cutaneous neoplasms

ASSUNTO(S)

anatomia patologica e patologia clinica anÃlise digital histoquÃmica neoplasias cutÃneas

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